Modified technique for spermatogonial stem cell transplantation into the seminiferous tubules in mouse model
نویسندگان
چکیده
منابع مشابه
Cell-cycle-dependent Colonization of Mouse Spermatogonial Stem Cells After Transplantation into Seminiferous Tubules
Spermatogonial stem cells (SSCs) migrate to the niche upon introduction into the seminiferous tubules of the testis of infertile animals. However, only 5-10% of the transplanted cells colonize recipient testes. In this study, we analyzed the impact of cell cycle on spermatogonial transplantation. We used fluorescent ubiquitination-based cell cycle indicator transgenic mice to examine the influe...
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objective: testicular cell transplantation has been widely used to investigate the restoration of fertility in rodent models. in this research we apply transplantation as a treatment method in the cryptorchid model and compare this method with orchidopexy, which is the routine treatment for this problem. we studied the controversial effects of treatment on the number of germ cells and other mor...
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Our previous study demonstrated that human umbilical cord mesenchymal stem cells (HUMSCs) were capable of differentiation into germ cells in vitro. To assess this potential in vivo, HUMSCs were microinjected into the lumen of seminiferous tubules of immunocompetent mice, which were treated with busulfan to destroy endogenous spermatogenesis. Bromodeoxyuridine labeling studies demonstrated that ...
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Belgian Malinois (BM), one of the excellent military dog breeds in South Korea, is usually castrated before sexual maturation. Therefore, the transfer of their genetic features to the next generation is difficult. To overcome this, testicular cells from 4-month-old BMs were frozen. Testicular cells were thawed after 3 months and cultured in StemPro-34 medium. Spermatogonial stem cell (SSC) char...
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S permatogonial stem cell (SSC) transplantation is a promising technique to circumvent sterility in prepubertal boys undergoing gonadotoxic treatments. While the cryopreservation of spermatogonial stem cells is being introduced in clinical practices worldwide, a lot of unanswered questions remain regarding their eventual transplantation. In this paper autologous and allogeneic SSC transplantati...
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ژورنال
عنوان ژورنال: Systems Biology in Reproductive Medicine
سال: 2012
ISSN: 1939-6368,1939-6376
DOI: 10.3109/19396368.2012.738762